Cytochrome C and Caspase-3/7 are Involved in Mycophenolic Acid-induced Apoptosis in Genetically Engineered PC12 Neuronal Cells Expressing the p53 Gene

نویسندگان

  • Hassan Malekinejad Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
  • Johanna Fink-Gremmels Department of Veterinary, Pharmacology, Pharmacy and Toxicology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 104, 3508 TD Utrecht, The Netherlands.
  • Masumeh Moradi Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
چکیده مقاله:

Mycophenolic acid (MPA) is the active metabolite of mycophenolate mofetil. This study designed to investigate the mechanism of cytotoxicity of MPA on the genetically engineered PC12 Tet Off (PTO) neuronal cells with p53 gene. Alamar Blue (AB) reduction showed concentration-dependent cytotoxicity of MPA on PTO cells with IC50 value of 32.32 ± 4.61 mM. The reactive oxygen species (ROS) generation following exposing the cells to MPA showed a significant (p < 0.05) increase in the ROS production and in a concentration-dependent fashion. Involvement of Caspase 3/7 proteases and Cytochrome C release in the induction of DNA fragmentation are all hallmarks of MPA-induced apoptosis in PTO cells. Our data suggest that MPA exerts an apoptotic effect on PTO cells. Moreover, the apoptotic effect of MPA attribute to the elevation of ROS generation by which might trigger the cytochrome C release and the activation of Caspase 3/7 that ultimately results in DNA fragmentation.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

cytochrome c and caspase-3/7 are involved in mycophenolic acid-induced apoptosis in genetically engineered pc12 neuronal cells expressing the p53 gene

mycophenolic acid (mpa) is the active metabolite of mycophenolate mofetil. this study designed to investigate the mechanism of cytotoxicity of mpa on the genetically engineered pc12 tet off (pto) neuronal cells with p53 gene. alamar blue (ab) reduction showed concentration-dependent cytotoxicity of mpa on pto cells with ic50 value of 32.32 ± 4.61 mm. the reactive oxygen species (ros) generation...

متن کامل

Cytochrome C and Caspase-3/7 are Involved in Mycophenolic Acid- Induced Apoptosis in Genetically Engineered PC12 Neuronal Cells Expressing the p53 gene

Mycophenolic acid (MPA) is the active metabolite of mycophenolate mofetil. This study designed to investigate the mechanism of cytotoxicity of MPA on the genetically engineered PC12 Tet Off (PTO) neuronal cells with p53 gene. Alamar Blue (AB) reduction showed concentration-dependent cytotoxicity of MPA on PTO cells with IC50 value of 32.32 ± 4.61 μM. The reactive oxygen species (ROS) generation...

متن کامل

Genetically Engineered Mesenchymal Stem Cells Stably Expressing Green Fluorescent Protein

Objective(s) Mesenchymal stem cells (MSCs) are nonhematopoietic stromal cells that are capable of differentiating into and contribute to the regeneration of mesenchymal tissues. Human mesenchymal stem cells (hMSCs) are ideal targets in cell transplantation and tissue engineering. Enhanced green fluorescent protein (EGFP) has been an important reporter gene for gene therapy. The aim of this stu...

متن کامل

Resveratrol inhibits β-amyloid-induced neuronal apoptosis via regulation of p53 acetylation in PC12 cells

The natural product resveratrol possesses diverse biological activities, including anti‑inflammatory, anti‑oxidant, anti‑cancer and anti‑aging effects in multiple organisms. The neuroprotective role of resveratrol has recently been reported in a cell model of amyloid (A)β(25‑35)‑induced neurotoxic injury using PC12 cells. However, the pathomechanism by which resveratrol inhibits neuronal apopto...

متن کامل

genetically engineered mesenchymal stem cells stably expressing green fluorescent protein

objective(s) mesenchymal stem cells (mscs) are nonhematopoietic stromal cells that are capable of differentiating into and contribute to the regeneration of mesenchymal tissues. human mesenchymal stem cells (hmscs) are ideal targets in cell transplantation and tissue engineering. enhanced green fluorescent protein (egfp) has been an important reporter gene for gene therapy. the aim of this stud...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 13  شماره 1

صفحات  191- 198

تاریخ انتشار 2014-01-01

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023